PK Y}X N$ N$ N Antimicrobial Activity Database -42-records-20181026_0327-comma_separated.csvUT K¡fK¡fBarcode,"Organisum 1","Extract Zone 1","Negitive Control Zone 1","Positive Control Zone 1","Quantity of BMP Extract loaded (ug)","Organism 2","Extract Zone 2","Negitive Control Zone 2","Positive Control Zone 2","Organism 3","Extract Zone 3","Negitive Control Zone 3","Positive Control Zone 3","Positive Control Used","Negitive Control Used","Additional Comments" 20180006,"M. luteus",10,6,38,1,"B. subtilis",6,6,30,"A. baumannii",6,6,13,Gentamicin,Methanol, 20180011,"M. luteus",14,6,38,1,"B. subtilis",6.5,6,30,"A. baumannii",6,6,13,Gentamicin,Methanol, 20180012,"M. luteus",6,6,38,1,"B. subtilis",6,6,30,"A. baumannii",6,6,13,Gentamicin,Methanol, 20180013,"M. luteus",6,6,38,1,"B. subtilis",6,6,30,"A. baumannii",6,6,13,Gentamicin,Methanol, 20180006,"K. pneumoniae",6,6,12,10,,,,,,,,,"gentamicin ",methanol, 20180011,"K. pneumoniae",6,6,12,10,,,,,,,,,"gentamicin ",methanol, 20180012,"K. pneumoniae",6,6,12,10,,,,,,,,,"gentamicin ",methanol, 20180013,"K. pneumoniae",6,6,12,10,,,,,,,,,"gentamicin ",methanol, 20180022,"S. aureus ATCC 25923",9,6,27,10,"E. coli ATCC 25922",6,6,24,,,,,Gentamicin,Methanol,"
10 microlitres of extract was placed on the disk. The above assumes that 10 microlitres is equivalent to 10 micrograms.Â
" 20180012,"A. baumannii",6,6,12,5,"M. luteus",8,9,21,,,,,"Gentamicin ",Methanol, 20180011,"A. baumannii",6,6,12,5,"M. luteus",11,9,21,,,,,"Gentamicin ",Methanol, 20180013,"A. baumannii",6,6,12,5,"M. luteus",10,9,21,,,,,"Gentamicin ",Methanol, 20180006,"A. baumannii",6,6,12,5,"M. luteus",11,9,21,,,,,"Gentamicin ",Methanol, 2018006,"E. coli ATCC 25922",6,6,23,10,"S. aureus ATCC 25923",6,6,30,"M. luteus",7,7,30,"Gentamicin ","Methanol ", 2018011,"B. subtilis",6,6,27,10,,,,,,,,,Gentamicin,Methanol, 20180006,"M. luteus",42,6,0,5,"K. pneumoniae",6,6,0,,,,,N/A,Methanol,"Massive inhibition zones were found with the M.luteus organism where none were found with K.pneumoniae.
No Gentomycin discs were used for a positive control.
Unfortunately M.luteus plate had an incident with excited butter fingers and ended up on the ground :(
large inhibition zone was found with the M.luteus organism where none were found with K.pneumoniae.
No Gentamicin discs were used for a positive control.
Unfortunately M.luteus plate had an incident with excited butter fingers and ended up on the ground :(
No inhibition zones were found with the M.luteus and K.pneumoniae organismÂ
No Gentamicin discs were used for a positive control.
Unfortunately M.luteus plate had an incident with excited butter fingers and ended up on the ground :(
No inhibition zones were found with the M.luteus and K.pneumoniae organismÂ
No Gentamicin discs were used for a positive control.
Unfortunately M.luteus plate had an incident with excited butter fingers and ended up on the ground :(
A 5.5mm measurement indicates that there was no observable activity for that assay, instead of writing 0mm the size of the disk (5.5mm) was noted down to account for possible activity beneath the surface of the disk.Â
Images below:
- E.coli on the left:Â Labelled area ""T1"" (the green disk) shows the sterile disk impregnanted with 20180022 extract. When placed on a lawn of E. coli no observable activity was recorded. To its left in anti-clockwise order are the controls Gentamicin, Sterile water and methanol.Â
- S. aureus on the right: Labelled area ""T1"" (the green disk) shows the sterile disk impregnanted with 20180022 extract. When placed on a lawn of S. aureus some activity was observed, if you look closely there is a 'halo' or 'haze' of colonies surrounding that disk in the clearing zone. To its left in anti-clockwise order are the controls Gentamicin, Sterile water and methanol.Â
Note: 'T2' in the image is a different plant extract - 20180025. The information for T2 are in a separate entry.Â
A 5.5mm measurement indicates that there was no observable activity for that assay, instead of writing 0mm the size of the disk (5.5mm) was noted down to account for possible activity beneath the surface of the disk.Â
Images below:
- E.coli on the left: Labelled area ""T2"" (the yellow disk) shows the sterile disk impregnanted with 20180025 extract. When placed on a lawn of E. coli no observable activity was recorded. To its right in clockwise order are the controls methanol, sterile water and Gentamicin. Â
- S. aureus on the right: Labelled area ""T2"" (the yellow disk) shows the sterile disk impregnanted with 20180025 extract. When placed on a lawn of S. aureus some activity was observed. To its right in clockwise order are the controls methanol, sterile water and Gentamicin. Â
Note: 'T1' in the image is a different plant extract - 20180022. The information for T1 is in a separate entry
The sizes of all disks were measured (6mm) and included as antimicrobial activity underneath the disk was unknown. All 6mm measurements above indicate no growth inhibition.
No clear inhibition of growth, with colony growth visible to the edge of the disks (except the positive control on the M. luteus plate).
" 20180027,"M. luteus",6,6,33,10,"Candida albicans",6,6,6,,,,,"Gentamicin (CN10)","Methanol & Sterile Water","The sizes of all disks were measured (6mm) and included as antimicrobial activity underneath the disk was unknown. All 6mm measurements above indicate no gowth inhibition.
It should be noted that there was changed activity of growth to one side of the 20180027 BMP Disk on the M. luteus plate. It can't be described as full inhibition, but there was a noticeable lessening of growth in a 7mm circle on the left side of the disk, though this may be explained as possible movement of the disk on the agar during the incubation period, as it does appear very similar to the shape and size of the disk.
No other clear inhibition zones were clear, with growth to the sides of the other disks (except the positive control on M. luteus of course).
" 20180021,"Salmonella Typhimurium",0,0,25,10,"K. pneumoniae",0,0,22,,,,,Gentamicin,Methanol, 20180027,"Salmonella Typhimurium",0,0,25,10,"K. pneumoniae",0,0,22,,,,,Gentamicin,Methanol, 20180021,"B. subtilis",6,6,30,10,"P. aeruginosa",6,6,24,,,,,Gentamicin,Methanol, 20180027,"B. subtilis",6,6,30,10,"P. aeruginosa",6,6,24,,,,,Gentamicin,Methanol, 25922,"E. coli ATCC 25922",6,6,15,10,N/A,,,,N/A,,,,"E. coli",methanol, 20180021,"S. aureus ATCC 25923",6,6,14,10,"M. luteus",6,6,17,,,,,gentamicin,"methanol ", PKÜ=êJN$ N$ PK Y}X f f C Plant Extract Database-19-records-20181026_0327-comma_separated.csvUT K¡fK¡fBarcode,"Mass of Dried Plant Sample","Mass of Extract (Total)","Mass of Extract (Toxicology)","Mass of Extract (Antimicrobial Testing)","Antimicrobial Activity","Additional Comments" 20180011,2.08,0.14,0.07,0.07,"Some Antimicrobial Activity", 20180024,1.514,0.095,0.016,0.079,"No Antimicrobial Activity","There is an area of growth inhibition around the 'methanol control' disk. Both of the sample disks showed no growth inhibition.Â
" 20180009,2.039,0.3864,0.2075,0.1789,"No Antimicrobial Activity","In terms of antimicrobial activity, if this sample has any properties at all they extend only to the size of the disk and thus only inhibit the growth a little, but not enough to have a proper zone. It appears that somewhere during processing the disk slipped to the left, as you can see circular markings in the space to the right of the disk that are the same size as the disk.Â
" 20180007,2.1818,0.2971,0.1196,0.1775,"No Antimicrobial Activity", 20180021,2.0051,15.9793,6.7989,9.1804,"No Antimicrobial Activity", 20180027,2.0086,0.7064,0.1609,0.5455,"No Antimicrobial Activity","Agar plate positions
Left Quadrant- Methanolic Extract
Right Quadrant - Methanol Control
Top Quadrant - Aqueous Control
Bottom Quadrant - Aqueous Extract
Less than 1mm of inhibition shown on methanolic sample, and possible inhibition suspected on the methanol control.
HPLC used 0.1% Ammonium Acetate rather than Formic acid.
1mm Antimicrobial growthÂ
" 20180014,1.61,0.37,0.23,0.14,"Some Antimicrobial Activity", 20180013,1.89,0.2,0.1,0.1,"Some Antimicrobial Activity", 20180006,2,0.05,0.06,0.06,"Some Antimicrobial Activity", 0,1.514,0.095,0.016,0.079,"No Antimicrobial Activity", 20180021,2.0051,15.9793,6.7989,9.1804,"No Antimicrobial Activity","Not sure if both partners were supposed to upload their data,
So I uploaded as well :-)Â
Luke
" 20180022,2.143,0.293,0.1515,0.1415,"No Antimicrobial Activity", 20180044,2.5,6,0.01,0.12,"No Antimicrobial Activity","No image of disk taken.